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  Zanco J Med Sci:  Dec. 2017; 21 (3): 1943-1952

Evaluation of salivary calcium, phosphorus and alkaline phosphatase level in children (4-6 years) with nursing caries in Erbil City

Zana Qadir Omer*, Baydaa Adnan Khoshnaw **

 

* Department of Peado, Orthod & Preventive, College of Dentistry, Hawler Medical University, Erbil, Iraq.

** Erbil health Directorate, Erbil, Iraq.
 


Abstract

Background and objective: Nursing caries is a complex and severe form of tooth decay that affects a child’s teeth and leads to severe pain, teeth loss, and psychological problems. This type of decay is caused by primary factors (host, cariogenic bacteria, fermentable carbohydrate and the time) and other secondary factors. Besides, the dental caries influence by the role of saliva as a defense system. These defense systems include clearance, buffering, antimicrobial agents, and calcium and phosphate delivery for remineralization. This study aimed to find out the relationship between calcium, inorganic phosphorus and alkaline phosphatase of unstimulated saliva in children with nursing caries.

Methods: The sample included 374 children aged 4-6 years; 324 were the study group (with nursing caries) while 50 were control group (caries-free). The case sheet and questionnaire included the child's feeding habits and oral hygiene. A 1.5-2 ml of saliva was collected from the selected children using spitting technique. The method included dental examination for the recording of the dmft and dmfs indices, and saliva analysis to determine the level of salivary calcium, phosphorus and alkaline phosphatase in control and study groups. 

Results: The study group children demonstrated a statistically highly significant (P ≤0.01) higher saliva calcium (5.229 ± 0.963 mg/dl) and alkaline phosphatase concentrations (6.321 ± 1.792 KAU/dl) than the control group (4.968± 0.757mg/dl, 5.384 ± 2.119 KAU/dl), respectively. The same group demonstrated a statistically highly significant (P ≤0.01) lower inorganic phosphorus concentration (10.991 ± 1.376 mg/dl) than the control group (11.961 ± 1.484 mg/dl). The study group showed a lower percentage of breastfeeding (28.4%) in comparison to the control group (42.0%). Concerning mix-feeding, the study group showed the higher percentage (45.4%) in comparison to the control group (32.0%). The bottle-fed found to be equal in both groups, there was a statistically non-significant difference between the study and control group.

Conclusion: Salivary calcium and alkaline phosphatase level have a major role in causing nursing caries in children. On the other hand, salivary inorganic phosphorus level showed a negative correlation with caries activity (dmfs). The type of infant feeding has no effect on the children with nursing caries.

Keywords: Saliva; Nursing caries; Calcium; Alkaline phosphatase; dmft; dmfs.

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Reference

1. Oliveira LB, Sheiham A, BÖncker M. Exploring the association of dental caries with factors and nutritional status in Brazilian preschool children. Eur J Oral Sci 2008; 116:37-43.

2. Dye BA, Tan S, Smith C, Lewis BG, Barker LK, Thornton-Evans G. Trends on oral health status; United States, 1988-1994 and 1999-2004. Vital and Health Statistics 2007; 11(248):1-92.

3. Hurlbutt M.’ Novy B. Young D.Dental caries: A PH- mediated disease. CDHAJ 2010; 25(1):9-14.

4. McDonald R, Avery D, Dean J. Dentistry for the child and adolescent. 8th ed. Maryland: Mosby Elsevier; 2004.

5. Hussein B. An assessment of concentrations of salivary calcium, phosphate and alkaline phosphatase activity with dietary habits analysis in children with rampant caries. MSc thesis in preventive Dentistry. University of Baghdad. College of Dentistry; 2002.

6. Namiq V. Early childhood caries, prevalence, severity, Treatment Needs and Risk factors in Preschool Children of Erbil city. MSc thesis. Hawler Medical University. College of Dentistry; 2010.

7. Tinanoff N. Introduction to the Early Childhood Caries Conference: Initial description and current understanding. Community Dent Oral Epidemiol 1998; 26:5-7.

8. World Health Organization. Oral health surveys Basic Methods. 3rd ed. World Health Organization. Geneva: WHO; 1987. 

9. Solderling E. Practical aspects of salivary analysis In Tenovuo J.O. Human saliva: clinical chemistry and microbiology. Florida. CRC Press 1989; l(1):1-24.

10. Anderson P, Hector MP, Rampersad MA. Critical pH in resting and stimulated whole saliva in groups children and adults. Int J pediatric Dent 2001; 11:266-73.

11. Murad-Patel R, Varman SSuragimath G, Zope S. Estimation and Comparison of Salivary Calcium, Phosphorous, Alkaline Phosphatase and pH Levels in Periodontal Health and Disease: A Cross-sectional Biochemical Study. J Clin Diagn Res 2016; 10(7):58-61.

12. Gamst OK, Try K. Zinc benefits. Scand J Clin Lab Invest 1980; 40:483-6.

13. Kind PR, King EJ. Estimation of plasma phosphatase by determination of hydrolyzed phenol with amino-anti- antipyrine, J Clin Path 1954; 7:322-6.

14. Belfield A, Goldberg DM. Revised assay for serum phenyl phosphatase activity using 4-amino- antipyrine. Enzyme 1971; 12:561-73.

15. Hurlbutt M, Novy B, Young D. Dental caries: A PH- mediated disease. CDHAJ 2010; 25(1):9-14.

16. Soames JV, Southam JC. Oral pathology. 4th ed. Oxford: Oxford University Press; 2005. p.84-106.

17. Elizarova VM, Petrovich IA. Calcium homeostasis disorder in children with multiple carries. Stomatology (Mosk) 2002; 1:63-6.

18. Cornejo LS, Brunotto M, Hilas E. Salivary factors associated to the prevalence and increase of dental caries in rural schoolchildren. Res Saud Public 2008; 42:19-25.

19. Kaur A, Kwatra KS, Kamboj P. Evaluation of non- microbial salivary caries activity parameters and salivary biochemical indicators in predicting dental caries. Ind Soci Ped. Prev. Dent J 2012; 30(3):212-7.

20. Pollard MA, Higham SM, Curzon ME, Edgar WM. Acid anion profiles in dental plaque following consumption of cereal-based foods and fruits. Eur J Oral Sci. 1996; 104(5-6):535-9.

21. Dawes C. Factors influencing salivary flow rate and composition. In: Edge W O, Mullane D edit. Saliva and oral Health. 2nd ed. London: Thanet Ltd; 1996. P:27-42.

22. Prabhakar AR, Shubha AB, Mahantesh T. Estimation of calcium, phosphate and Alph Amylase Concentration in the stimulated whole saliva of children with different caries status: A comparative study. Malaysian Dent J 2008; 29(1):6-13.

23. Damle SG, Vidya I, Yadav R, Rhattal H, loomba A. Quantitive determination of inorganic constituents in saliva and their relationship with dental caries experience in children. Dentistry 2012; 2:131.

24. Mahjoub S, Ghasempour M, Mohammadi A. Salivary Alkaline phosphatase and Inorganic phosphorus concentration in children with different Dental Caries. TBUMS 2007; 9:23-8.

25. Vijayaprasad KE, Ravichandran KS., Vasa A, Suzan S. Relation of salivary calcium, phosphorus and alkaline phosphates with the incidence of dental caries in children. J Indian Soc Pedod Prev Dent 2010; 28:156-61.

26. Dabra S, Singh P. Evaluating the levels of salivary alkaline and acid phosphatase activities as biochemical markers for periodontal disease: A case series. Dent Res J (Isfahan) 2012; 9(1):41-5.

27. Gandhy M, Damle SG. Relation of salivary inorganic phosphor and alkaline phosphatase to the dental caries status in children. J Indian Soc Pedod Prev Dent 2003; 21(4):135-8.

28. Al-Obaidi WM, Al-Obaidi WA. Severity of dental caries in relation to salivary parameters and inorganic composition among group of 22-23 years old adult in Baghdad city. J Bagh Coll Dentistry 2010; 22(2):118-22.

29. Oulis CJ, Perdouses ED, Vadiakas G, Lygidakis NA. Feeding practices of Greek children with and without nursing caries. Ped Dent 1999; 20(7):409-16.

30. Schroth RJ, Whalen JC, Lekic C, Moffatt MEK. Prevalence of caries among pre-school aged children in Northen Manitoba Community. J Cand Den Assoc 2005; 71(1):27.

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